Sea turtle monitoring // Pervasive genome transcription is initiated from distinct promoters
Topics discussed included:
1) How fishermen cooperate with scientists to enable location monitoring (tag and release) and genetic sampling of sea turtles (caught accidentally in fishing nets), yielding data about sea turtle movents in the oceans. Non-profit organisations are key to this cooperation. We speculated about what would motivate busy fishermen to bring these turtles to port for the scientific studies....
2) A recent paper illuminating some of the "dark matter" pervasively transcribed in the human genome:
Genomic organization of human transcription initiation complexes
by Bryan J. Venters and B. Franklin Pugh
published online last week in Nature
Using ChIP-exo followed by high-throughput DNA-seq, these authors identified about 160,000 transcription initiation complexes bound to specific site across the human genome. The vast majority of these complexes were bound to sites containing 4 core promoter elements: upstream TFIIB recognition element (BRE-u), TATA, downstream TFIIB recognition element (BREd), and initiator element (INR), in highly constrained positions. All but the INR also reside at Pol III promoters, where TATA-binding protein (TPB) makes similar contacts as at Pol II prmoters.
These and other data reported here seem to pretty persuasively indicate that the still-mysterious pervasive transcription of the human genome is generally promoter-specific rather than randomly initiated.
Topics discussed included:
1) How fishermen cooperate with scientists to enable location monitoring (tag and release) and genetic sampling of sea turtles (caught accidentally in fishing nets), yielding data about sea turtle movents in the oceans. Non-profit organisations are key to this cooperation. We speculated about what would motivate busy fishermen to bring these turtles to port for the scientific studies....
2) A recent paper illuminating some of the "dark matter" pervasively transcribed in the human genome:
Genomic organization of human transcription initiation complexes
by Bryan J. Venters and B. Franklin Pugh
published online last week in Nature
Using ChIP-exo followed by high-throughput DNA-seq, these authors identified about 160,000 transcription initiation complexes bound to specific site across the human genome. The vast majority of these complexes were bound to sites containing 4 core promoter elements: upstream TFIIB recognition element (BRE-u), TATA, downstream TFIIB recognition element (BREd), and initiator element (INR), in highly constrained positions. All but the INR also reside at Pol III promoters, where TATA-binding protein (TPB) makes similar contacts as at Pol II prmoters.
These and other data reported here seem to pretty persuasively indicate that the still-mysterious pervasive transcription of the human genome is generally promoter-specific rather than randomly initiated.
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